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Table of Contents

  1. Chapter 1 Introduction

    1. Using this Book with Your Curriculum

    2. Planning and Scheduling

    3. Work Areas

    4. Maintaining a Laboratory Notebook

  2. Chapter 2 Equipping a Home Biology Laboratory

    1. Microscopes and Accessories

    2. Microscope Accessories

    3. Culturing Equipment and Supplies

    4. Histology Equipment and Supplies

    5. General Laboratory Equipment

    6. Major Equipment

    7. Prepared Slides

    8. Specimens

  3. Chapter 3 Laboratory Safety

    1. Prepare Properly

    2. Dress Properly

    3. Avoid Laboratory Hazards

    4. Don’t Do Stupid Things

  4. Chapter 4 Using a Microscope

    1. Equipment and Materials

    2. Background

    3. Procedure I-1-1: A Microscope Tour

    4. Build a Darkfield Apparatus from Pocket Change

    5. Review Questions

  5. Chapter 5 Mounting Specimens

    1. Equipment and Materials

    2. Background

    3. Procedure I-2-1: Making Wet Mounts

    4. Procedure I-2-2: Making Smear Mounts

    5. Procedure I-2-3: Making Hanging Drop Mounts

    6. Procedure I-2-4: Making Sectional Mounts

    7. Review Questions

  6. Chapter 6 Staining

    1. Equipment and Materials

    2. Background

    3. Procedure I-3-1: Simple Staining

    4. Procedure I-3-2: Gram Staining

    5. Review Questions

  7. Chapter 7 Building and Observing Microcosms

    1. Equipment and Materials

    2. Background

    3. Procedure II-1-1: Gathering Materials

    4. Procedure II-1-2: Building Aquarium Microcosms

    5. Procedure II-1-3: Building Winogradsky Column Microcosms

    6. Procedure II-1-4: Observing Winogradsky Column Microcosms

    7. Review Questions

  8. Chapter 8 Observing Succession in Aquarium Microcosms

    1. Equipment and Materials

    2. Background

    3. Procedure II-2-1: Observe Succession in Microcosms

    4. Review Questions

  9. Chapter 9 Observing the Effects of Pollution in Microcosms

    1. Equipment and Materials

    2. Background

    3. Procedure II-3-1: Build Polluted Microcosms

    4. Procedure II-3-2: Observe Succession in Polluted Microcosms

    5. Review Questions

  10. Chapter 10 Acids, Bases, and Buffers

    1. Equipment and Materials

    2. Background

    3. Procedure III-1-1: Percentage and Molar Concentrations

    4. Procedure III-1-2: Effect of Concentration on pH

    5. Procedure III-1-3: pH of Household Materials

    6. Procedure III-1-4: Buffers

    7. Review Questions

  11. Chapter 11 Carbohydrates and Lipids

    1. Equipment and Materials

    2. Background

    3. Procedure III-2-1: Investigating Sugars

    4. Procedure III-2-2: Investigating Starches

    5. Procedure III-2-3: Investigating Lipids

    6. Review Questions

  12. Chapter 12 Proteins, Enzymes, and Vitamins

    1. Equipment and Materials

    2. Background

    3. Procedure III-3-1: Investigating Proteins

    4. Procedure III-3-2: Investigating Enzyme Catalysis

    5. Procedure III-3-3: Assaying Vitamin C Concentration in Urine

    6. Review Questions

  13. Chapter 13 Coacervates

    1. Equipment and Materials

    2. Background

    3. Procedure III-4-1: Prepare and Observe a Coacervate

    4. Review Questions

  14. Chapter 14 Extracting, Isolating, and Visualizing DNA

    1. Equipment and Materials

    2. Background

    3. Procedure III-5-1: Extracting and Visualizing DNA

    4. Review Questions

  15. Chapter 15 Build a Gel Electrophoresis Apparatus

    1. Equipment and Materials

    2. Background

    3. Procedure III-6-1: Making the Gel Casting Container and Comb

    4. Procedure III-6-2: Assemble the Apparatus

  16. Chapter 16 Simulated DNA Separation by Gel Electrophoresis

    1. Equipment and Materials

    2. Background

    3. Procedure III-7-1: Prepare Running Buffer

    4. Procedure III-7-2: Prepare and Cast the Gel

    5. Procedure III-7-3: Load and Run the Dye Specimens

    6. Review Questions

  17. Chapter 17 Chlorophyll and Photosynthesis

    1. Equipment and Materials

    2. Background

    3. Procedure IV-1-1: Observing Carbon Dioxide Uptake

    4. Procedure IV-1-2: Determining the Effect of Light Intensity on Photosynthesis

    5. Procedure IV-1-3: Chromatography of Plant Pigments and Locating Cellular Energy Reserves

    6. Review Questions

  18. Chapter 18 Investigating Osmosis

    1. Equipment and Materials

    2. Background

    3. Procedure IV-2-1: Observing Osmosis in Chicken Eggs

    4. Review Questions

  19. Chapter 19 Investigating Cell Division

    1. Equipment and Materials

    2. Background

    3. Procedure IV-3-1: Observing Mitosis

    4. Review Questions

  20. Chapter 20 Sampling Plant Populations in a Community

    1. Equipment and Materials

    2. Background

    3. Procedure V-1-1: Choosing and Preparing the Survey Area

    4. Procedure V-1-2: Surveying the Plant Community

    5. Procedure V-1-3: Performing a Population Count

    6. Review Questions

  21. Chapter 21 Observing the Effect of Rhizobia on Plant Growth

    1. Equipment and Materials

    2. Background

    3. Procedure V-2-1: Grow Lima Beans With and Without Rhizobia

    4. Review Questions

  22. Chapter 22 Air Pollution Testing

    1. Equipment and Materials

    2. Background

    3. Procedure V-3-1: Building Particle Traps

    4. Procedure V-3-2: Positioning and Exposing the Particle Traps

    5. Procedure V-3-3: Counting and Identifying Particles

    6. Review Questions

  23. Chapter 23 Soil and Water Pollution Testing

    1. Equipment and Materials

    2. Background

    3. Procedure V-4-1: Obtaining Soil and Water Specimens

    4. Procedure V-4-2: Testing the Reagents

    5. Procedure V-4-3: Making Boron Concentration Comparison Standards

    6. Procedure V-4-4: Testing Specimens for Boron

    7. Review Questions

  24. Chapter 24 Exploring Mendelian Genetics

    1. Equipment and Materials

    2. Background

    3. Procedure VI-1-1: Testing Subjects for the Ability to Taste PTC

    4. Procedure VI-1-2: Charting Inheritance of PTC-Tasting Ability

    5. Review Questions

  25. Chapter 25 Observing Specialized Eukaryotic Cells

    1. Equipment and Materials

    2. Background

    3. Procedure VII-1-1: Observing Onion Epidermal and Elodea Leaf Cells

    4. Procedure VII-1-2: Comparing and Contrasting Eukaryotic Cells

    5. Review Questions

  26. Chapter 26 Preparing Culturing Media

    1. Equipment and Materials

    2. Background

    3. Procedure VII-2-1: Preparing Normal Saline and Nutrient Media

    4. Review Questions

  27. Chapter 27 Culturing Bacteria

    1. Equipment and Materials

    2. Background

    3. Procedure VII-3-1: Staining and Observing the Original Culture

    4. Procedure VII-3-2: Inoculating Plates and Tubes

    5. Procedure VII-3-3: Producing Pure Cultures

    6. Review Questions

  28. Chapter 28 Investigating Bacterial Antibiotic Sensitivity

    1. Equipment and Materials

    2. Background

    3. Procedure VII-4-1: Testing Antibiotic Sensitivity

    4. Procedure VII-4-2: Culturing an Antibiotic-Resistant Bacteria Strain

    5. Procedure VII-4-3: Retesting Sensitivity of the Resistant Strain

    6. Review Questions

  29. Chapter 29 Investigating Protista

    1. Equipment and Materials

    2. Background

    3. Procedure VIII-1-1: Observing Spirogyrae

    4. Procedure VIII-1-2: Observing Euglenae

    5. Procedure VIII-1-3: Observing Amoebae

    6. Procedure VIII-1-4: Observing Paramecia

    7. Review Questions

  30. Chapter 30 Investigating Fungi

    1. Equipment and Materials

    2. Background

    3. Procedure IX-1-1: Observing Zygomycota

    4. Procedure IX-1-2: Observing Ascomycota

    5. Procedure IX-1-3: Observing Basidiomycota

    6. Review Questions

  31. Chapter 31 Investigating Simple Plants: Mosses and Ferns

    1. Equipment and Materials

    2. Background

    3. Procedure X-1-1: Observing Moss Structures

    4. Procedure X-1-2: Observing Fern Structures

    5. Review Questions

  32. Chapter 32 Investigating Seed Plants

    1. Equipment and Materials

    2. Background

    3. Procedure X-2-1: Observing Germination of a Seed Plant

    4. Procedure X-2-2: Observing Root Structures

    5. Procedure X-2-3: Observing Stem Structures

    6. Procedure X-2-4: Observing Leaf Structures

    7. Procedure X-2-5: Observing Reproductive Structures

    8. Review Questions

  33. Chapter 33 Investigating Porifera and Cnidaria

    1. Equipment and Materials

    2. Background

    3. Procedure XI-1-1: Observing Porifera

    4. Procedure XI-1-2: Observing Cnidaria

    5. Review Questions

  34. Chapter 34 Investigating Platyhelminthes, Nematoda, and Annelida

    1. Equipment and Materials

    2. Background

    3. Procedure XI-2-1: Observing Platyhelminthes (Flatworms)

    4. Procedure XI-2-2: Observing Nematoda (Roundworms)

    5. Procedure XI-2-3: Observing Annelida (Segmented Worms)

    6. Review Questions

  35. Chapter 35 Investigating Arthropods

    1. Equipment and Materials

    2. Background

    3. Procedure XI-3-1: Observing and Comparing Arthropod Structures

    4. Procedure XI-3-2: Observing Insect Metamorphosis

    5. Review Questions

  36. Chapter 36 Investigating Vertebrate Tissues

    1. Equipment and Materials

    2. Background

    3. Procedure XI-4-1: Observing Epithelial Tissues

    4. Procedure XI-4-2: Observing Connective Tissues

    5. Procedure XI-4-3: Observing Muscle Tissues

    6. Procedure XI-4-4: Observing Nervous Tissues

    7. Review Questions